Polyethylene glycol improves current methods for circulating extracellular vesicle-derived DNA isolation
نویسندگان
چکیده
منابع مشابه
Current methods for the isolation of extracellular vesicles.
Extracellular vesicles (EVs), including microvesicles and exosomes, are nano- to micron-sized vesicles, which may deliver bioactive cargos that include lipids, growth factors and their receptors, proteases, signaling molecules, as well as mRNA and non-coding RNA, released from the cell of origin, to target cells. EVs are released by all cell types and likely induced by mechanisms involved in on...
متن کاملReady-made chromatography columns for extracellular vesicle isolation from plasma
Proteomic studies of circulating vesicles are hampered by difficulties in purifying vesicles from plasma and serum. Isolations are contaminated with high-abundance blood proteins that may mask genuine vesicular-associated proteins and/or simply provide misleading data. In this brief report, we explored the potential utility of a commercially available size exclusion chromatography column for ra...
متن کاملMicrofluidic isolation of cancer-cell-derived microvesicles from hetergeneous extracellular shed vesicle populations.
Extracellular shed vesicles, including exosomes and microvesicles, are disseminated throughout the body and represent an important conduit of cell communication. Cancer-cell-derived microvesicles have potential as a cancer biomarker as they help shape the tumor microenvironment to promote the growth of the primary tumor and prime the metastatic niche. It is likely that, in cancer cell cultures,...
متن کاملPrecipitation of DNA by polyethylene glycol and ethanol.
The use of polyethylene glycol (PEG) for precipitation of DNA has been described in the literature (1-6) . We present here conditions for precipitation of DNA by PEG that give improved recoveries of DNA so that precipitation by PEG is comparable to that by ethanol, in terms of recovery, over a wide range of DNA concentrations. The problem of precipitation of lowmolecular-weight DNA fragments by...
متن کاملStandardization of sample collection, isolation and analysis methods in extracellular vesicle research
The emergence of publications on extracellular RNA (exRNA) and extracellular vesicles (EV) has highlighted the potential of these molecules and vehicles as biomarkers of disease and therapeutic targets. These findings have created a paradigm shift, most prominently in the field of oncology, prompting expanded interest in the field and dedication of funds for EV research. At the same time, under...
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ژورنال
عنوان ژورنال: Journal of Translational Medicine
سال: 2019
ISSN: 1479-5876
DOI: 10.1186/s12967-019-1825-3